Coding

Part:BBa_M36760:Design

Designed by: Beatriz Collazo, Paul Hichwa, John Pluvinage   Group: Stanford BIOE44 - S11   (2012-12-04)


Channelrhodopsin-2 light-gated Ca2+ channel


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 197
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 197
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 282
    Illegal XhoI site found at 320
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 197
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 197
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added a e5'UTR-bG from Gene Designer from DNA 2.0, kozak consensus sequence including the start codon (gccaccatg) sequence and a stop codon (tag). No mutations were needed for repeats in the coding sequence.


Source

Optimized for expression and sequenced by the Deisseroth Lab at Stanford University. We obtained the ChR2 coding sequence from the Stanford Optogenetics Resource Center. http://www.stanford.edu/group/dlab/optogenetics/sequence_info.html

References